The experimental exhibition, entitled "Leonardo: Experience A Masterpiece," will invite visitors to delve into the Old Master's "The Virgin of the Rocks".
The secrets of the masterpiece will be unveiled through a wide range of multisensory experiences presented across four separate rooms.
One of them will invite museumgoers to step into an imagined chapel to contemplate how "The Virgin of the Rocks" would have appeared in the chapel of the Confraternity of the Immaculate Conception in the Milanese church of San Francesco Grande, where it was housed in the early 1500s.
Another room will detail the latest scientific findings underneath the masterpiece, pointing out how Leonardo da Vinci abandoned his initial designs for the angel featured in the painting as well as the Infant Christ.
In the abandoned composition, both figures were positioned higher up with the angel notably looking down on the Infant Christ.
"By its very nature, much of the research we do at the National Gallery takes place in closed studios, laboratories and libraries. This is an exciting opportunity to not only share our innovative findings, but also to invite the public to explore and engage with what we have found," Dr Caroline Campbell, the museum's director of collections and research, noted in a statement.
"Leonardo: Experience A Masterpiece" was created by the award-winning company 59 Productions, which has previously created the video design of the Opening Ceremony of the 2012 Olympic Games.
The exhibition will be on view from November 9 through January 12 in London, marking the National Gallery's contribution to celebrations for the 500th anniversary of Leonardo da Vinci's death.
Meanwhile, across the English Channel in Paris, the Louvre has made an offer to the owner of "Salvator Mundi" to display the world's most expensive painting as part of its blockbuster Leonardo da Vinci show.
The masterpiece has not been seen in public since it went under the hammer for a record-breaking $450 million at Christie's in November 2017.
In the meantime, discover a trailer for the National Gallery's upcoming exhibition, "Leonardo: Experience A Masterpiece":