Opening batsman Jason Roy has been ruled out of the rest of the T20 World Cup, the England and Wales Cricket Board announced on Monday.
Roy, 31, suffered a calf injury and collapsed to the ground while chasing a quick single in Saturday's 10-run loss to South Africa.
Despite their first defeat of the tournament, Eoin Morgan's side advanced to the semi-finals and Roy will be replaced in the squad by James Vince ahead of England's last-four match against New Zealand in Abu Dhabi on Wednesday.
Roy has joined left-arm fast bowler Tymal Mills on the injured list in another blow to England, who lost to the West Indies in the final of the 2016 tournament.
"I'm gutted to be ruled out of the World Cup," said Roy in an England statement. "It is a bitter pill to swallow."
"I will be staying on to support the boys and hopefully we can go all the way and lift that trophy," added the Surrey star, a key member of the England side that won the 2019 50-over World Cup.
"We're all gutted for you @JasonRoy20," said a tweet on the England Cricket account.
"We will carry on playing in the positive spirit that you embody. If anyone can come back stronger, it's you."
Roy had made 123 runs in five innings at an average of 30.75 and a strike-rate of 138.2 in a tournament at which his opening partnership with Jos Buttler has often provided England with an ideal launchpad.
There are a number of options for a replacement opening partner alongside Buttler including Jonny Bairstow, Liam Livingstone, Dawid Malan and Moeen Ali.
"It's always nice to have a confident Jason Roy who's playing well," said Moeen.
"But we've got guys who've been waiting in the wings for a long time, who are playing well in the nets, who are ready to come in and play well.
"You can bring in Vince, you can bring in (Sam) Billings, you can bring in a bowler, the great thing is there's loads of options and loads of combinations that we can go with," the all-rounder added.
England arrived in the Gulf without the injured Ben Stokes, Jofra Archer and Sam Curran before Mills broke down with a thigh strain.
Morgan's side are bidding to become the first men's team to hold cricket's two major global limited-overs titles simultaneously.