Scott Cummings has dropped out of the Scotland squad after picking up an injury in Sunday's Six Nations game against Ireland but Zander Fagerson is back after a four-week ban.
Lock Cummings and 21-year-old back Rufus McLean are the only two players to drop out of Gregor Townsend's squad following the 27-24 defeat in Edinburgh, which ended Scotland's Six Nations title hopes.
Cummings suffered a suspected hand fracture while fellow lock Jonny Gray sustained a shoulder injury.
Stand-off Finn Russell picked up a head knock, which looks likely to rule him out of Saturday's visit of Italy. However, Gray and Russell are both included in the 35-man squad.
Uncapped back-row forward Ally Miller and his Edinburgh team-mate, back Damien Hoyland, have been drafted in.
Fagerson is back in the camp after being banned following a red card in the narrow defeat against Wales last month.
Speaking on the injury situation after Sunday's narrow defeat, Townsend said: "Finn was removed from play with a concussion, so it's unlikely he will feature in six days' time."
Scotland are above only perennial strugglers Italy in the Six Nations table but have a game in hand over most of the sides above them.